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alpha Tubulin antibody [HL2162]

Recombinant antibody
Anti-alpha Tubulin antibody [HL2162] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638140
Anti-alpha Tubulin antibody [HL2162] used in Western Blot (WB). GTX638140
Anti-alpha Tubulin antibody [HL2162] used in Western Blot (WB). GTX638140
Anti-alpha Tubulin antibody [HL2162] used in Western Blot (WB). GTX638140
Anti-alpha Tubulin antibody [HL2162] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638140
Anti-alpha Tubulin antibody [HL2162] used in IHC (Paraffin sections) (IHC-P). GTX638140
Anti-alpha Tubulin antibody [HL2162] used in IHC (Paraffin sections) (IHC-P). GTX638140
Anti-alpha Tubulin antibody [HL2162] used in Western Blot (WB). GTX638140
Anti-alpha Tubulin antibody [HL2162] used in Western Blot (WB). GTX638140
Anti-alpha Tubulin antibody [HL2162] used in Western Blot (WB). GTX638140

Cat. No. GTX638140

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2162

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish, Rabbit, Cat, Dog, Hamster, African green monkey, E. coli
Package
100 μl ($399),
25 μl ($169)
See all alpha Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human alpha Tubulin.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-alpha Tubulin antibody [HL2162] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638140

GTX638140 ICC/IF Image

alpha Tubulin antibody [HL2162] detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin stained by alpha Tubulin antibody [HL2162] (GTX638140) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-alpha Tubulin antibody [HL2162] used in Western Blot (WB). GTX638140

GTX638140 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [HL2162] (GTX638140) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [HL2162] used in Western Blot (WB). GTX638140

GTX638140 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [HL2162] (GTX638140) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [HL2162] used in Western Blot (WB). GTX638140

GTX638140 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [HL2162] (GTX638140) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [HL2162] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638140

GTX638140 ICC/IF Image

alpha Tubulin antibody [HL2162] detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin stained by alpha Tubulin antibody [HL2162] (GTX638140) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-alpha Tubulin antibody [HL2162] used in IHC (Paraffin sections) (IHC-P). GTX638140

GTX638140 IHC-P Image

alpha Tubulin antibody [HL2162] detects alpha Tubulin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
alpha Tubulin stained by alpha Tubulin antibody [HL2162] (GTX638140) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Tubulin antibody [HL2162] used in IHC (Paraffin sections) (IHC-P). GTX638140

GTX638140 IHC-P Image

alpha Tubulin antibody [HL2162] detects alpha Tubulin protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
alpha Tubulin stained by alpha Tubulin antibody [HL2162] (GTX638140) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Tubulin antibody [HL2162] used in Western Blot (WB). GTX638140

GTX638140 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [HL2162] (GTX638140) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [HL2162] used in Western Blot (WB). GTX638140

GTX638140 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [HL2162] (GTX638140) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [HL2162] used in Western Blot (WB). GTX638140

GTX638140 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [HL2162] (GTX638140) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations