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mTOR (phospho Ser2448) antibody

Orthogonal
Anti-mTOR (phospho Ser2448) antibody used in Western Blot (WB). GTX132803
Anti-mTOR (phospho Ser2448) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132803
Anti-mTOR (phospho Ser2448) antibody used in Western Blot (WB). GTX132803
Anti-mTOR (phospho Ser2448) antibody used in Western Blot (WB). GTX132803
Anti-mTOR (phospho Ser2448) antibody used in IHC (Paraffin sections) (IHC-P). GTX132803
Anti-mTOR (phospho Ser2448) antibody used in Western Blot (WB). GTX132803
Anti-mTOR (phospho Ser2448) antibody used in IHC (Paraffin sections) (IHC-P). GTX132803
Anti-mTOR (phospho Ser2448) antibody used in IHC (Paraffin sections) (IHC-P). GTX132803
Anti-mTOR (phospho Ser2448) antibody used in Western Blot (WB). GTX132803
Anti-mTOR (phospho Ser2448) antibody used in Western Blot (WB). GTX132803

Cat. No. GTX132803

Reference 15

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all mTOR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

289 kDa. ( Note )

Positive Control

293T (200 ng/ml EGF treatment for 0.5 hr) , NIH-3T3 (starvation for 16 hr and 200 ng/ml EGF treatment for 0.5 hr)

Predict Reactivity

Zebrafish, Sheep(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser2448 of human mTOR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886750

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mechanistic target of rapamycin kinase , FRAP , FRAP1 , FRAP2 , RAFT1 , RAPT1 , SKS

Cellular Localization

Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side

Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-mTOR (phospho Ser2448) antibody used in Western Blot (WB). GTX132803

GTX132803 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR (phospho Ser2448) antibody (GTX132803) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mTOR (phospho Ser2448) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132803

GTX132803 ICC/IF Image

mTOR (phospho Ser2448) antibody detects mTOR (phospho Ser2448) protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: mTOR (phospho Ser2448) stained by mTOR (phospho Ser2448) antibody (GTX132803) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-mTOR (phospho Ser2448) antibody used in Western Blot (WB). GTX132803

GTX132803 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR (phospho Ser2448) antibody (GTX132803) diluted at 1:1000.

Anti-mTOR (phospho Ser2448) antibody used in Western Blot (WB). GTX132803

GTX132803 WB Image

Orthogonal

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR (phospho Ser2448) antibody (GTX132803) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.

Anti-mTOR (phospho Ser2448) antibody used in IHC (Paraffin sections) (IHC-P). GTX132803

GTX132803 IHC-P Image

mTOR (phospho Ser2448) antibody detects mTOR (phospho Ser2448) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).
Sample: Paraffin-embedded human lung cancer.
mTOR (phospho Ser2448) stained by mTOR (phospho Ser2448) antibody (GTX132803) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-mTOR (phospho Ser2448) antibody used in Western Blot (WB). GTX132803

GTX132803 WB Image

Orthogonal

The data was published in the journal Aging (Albany NY) in 2019. PMID: 31831718

Anti-mTOR (phospho Ser2448) antibody used in IHC (Paraffin sections) (IHC-P). GTX132803

GTX132803 IHC-P Image

Orthogonal

The data was published in the journal Aging (Albany NY) in 2019. PMID: 31831718

Anti-mTOR (phospho Ser2448) antibody used in IHC (Paraffin sections) (IHC-P). GTX132803

GTX132803 IHC-P Image

The data was published in the journal Aging (Albany NY) in 2019. PMID: 31831718

Anti-mTOR (phospho Ser2448) antibody used in Western Blot (WB). GTX132803

GTX132803 WB Image

The data was published in the journal Aging (Albany NY) in 2019. PMID: 31831718

Anti-mTOR (phospho Ser2448) antibody used in Western Blot (WB). GTX132803

GTX132803 WB Image

The data was published in the journal Aging (Albany NY) in 2019. PMID: 31831718

REFERENCE

Application Reference

Zhong Y et al. Nutrients 2023; 15 (16) Diosgenin Targets CaMKK2 to Alleviate Type II Diabetic Nephropathy through Improving Autophagy, Mitophagy and Mitochondrial Dynamics.
Tian X et al. Chin Med 2023; 18 (1) : 79 Yangqing Chenfei formula alleviates silica-induced pulmonary inflammation in rats by inhibiting macrophage M1 polarization.
Yunjiao Shen et al. Authorea. 2023; Transformation of ginsenosides by Lactiplantibacillus plantarum MB11 fermentation: minor ginsenosides conversion and enhancement of anti-colorectal cancer activity
Application : WB
Parija M et al. Oncotarget 2023; 14 : 466-480 G-protein-coupled receptor 141 mediates breast cancer proliferation and metastasis by regulating oncogenic mediators and the p-mTOR/p53 axis.
Hashikawa-Hobara N et al. PeerJ 2021; 9 : e11720 CGRP overexpression does not alter depression-like behavior in mice.
Application : WB
Reactivity : Mouse
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REVIEW

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Application Reference
Zhong Y et al. Nutrients 2023; 15 (16) Diosgenin Targets CaMKK2 to Alleviate Type II Diabetic Nephropathy through Improving Autophagy, Mitophagy and Mitochondrial Dynamics.
Tian X et al. Chin Med 2023; 18 (1):79 Yangqing Chenfei formula alleviates silica-induced pulmonary inflammation in rats by inhibiting macrophage M1 polarization.
Yunjiao Shen et al. Authorea. 2023; Transformation of ginsenosides by Lactiplantibacillus plantarum MB11 fermentation: minor ginsenosides conversion and enhancement of anti-colorectal cancer activity
Application : WB
Parija M et al. Oncotarget 2023; 14:466-480 G-protein-coupled receptor 141 mediates breast cancer proliferation and metastasis by regulating oncogenic mediators and the p-mTOR/p53 axis.
Hashikawa-Hobara N et al. PeerJ 2021; 9:e11720 CGRP overexpression does not alter depression-like behavior in mice.
Application : WB
Reactivity : Mouse
Mori Y et al. FEBS Open Bio 2021; 11 (5):1382-1394 CD133 prevents colon cancer cell death induced by serum deprivation through activation of Akt-mediated protein synthesis and inhibition of apoptosis.
Application : WB
Reactivity : Human
Liu Z et al. Mol Med Rep 2021; 23 (2):1 MicroRNA?223?induced inhibition of the FBXW7 gene affects the proliferation and apoptosis of colorectal cancer cells via the Notch and Akt/mTOR pathways.
Application : WB
Reactivity : Human
Cheung CHY et al. J Biomed Sci 2020; 27 (1):75 ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer.
Wang H et al. J Cell Mol Med 2020; 24 (2):1399-1412 Long non-coding RNA (lncRNA) H19 induces hepatic steatosis through activating MLXIPL and mTORC1 networks in hepatocytes.
Li J et al. J Ethnopharmacol 2020; 257:112796 Effective-component compatibility of Bufei Yishen formula II inhibits mucus hypersecretion of chronic obstructive pulmonary disease rats by regulating EGFR/PI3K/mTOR signaling.
Application : WB, IHC-P
Reactivity : Rat
Li H et al. Neurotox Res 2020; 37 (4):996-1008 Aluminum-Induced Synaptic Plasticity Impairment via PI3K-Akt-mTOR Signaling Pathway.
Application : WB
Reactivity : Rat
Peng Q et al. Aging (Albany NY) 2019; 11 (23):11520-11540 Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes.
Application : WB
Reactivity : Human
Chen YL et al. Cancers (Basel) 2019; 11 (5) mTOR Inhibitors Can Enhance the Anti-Tumor Effects of DNA Vaccines through Modulating Dendritic Cell Function in the Tumor Microenvironment.
Application : WB
Reactivity : Mouse
Lin JT et al. Journal of Functional Foods 2018; 47:343-349 Protective effect and mechanism of Muntingia calabura Linn. fruit ethanolic extract against vascular endothelial growth factor production in nickel-stimulated hepatocellular carcinoma cells.
Application : WB
Reactivity : Human
Chen PH et al. PLoS One 2016; 11 (11):e0167096 the inhibition of microrna-128 on igf-1-activating mtor signaling involves in *-induced glioma cell apoptotic death.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169